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Next, students were introduced to the micropipette, an essential tool in any molecular
biology lab. They were taught the proper techniques for handling and using this
precision instrument, including how to measure and transfer small volumes of liquids
accurately. This skill is critical for various experiments, including the PCR process.
With their DNA samples prepared and their skills obtained to use the micropipette
developed, students carried out a Polymerase Chain Reaction (PCR) experiment. This
technique is used to amplify small segments of DNA and is a cornerstone of modern
molecular biology. Under the guidance of instructors, students set up their PCR
reactions, learning about the importance of primers, nucleotides, and DNA
polymerase in the process.
Following the PCR experiment, students performed electrophoresis to separate and
visualise the amplified DNA fragments. This technique allowed them to see the results
of their PCR experiments and understand how scientists analyse DNA samples. The
excitement was palpable as students observed the bands on the gel, indicating
successful amplification.
In addition to the hands-on activities, the camp featured several lectures on advanced
topics such as microsatellites, the detailed workings of PCR, and other molecular
biology techniques. These lectures provided the theoretical background needed to
understand the experiments, deepening the students' knowledge of genetics and
biotechnology.
Throughout the camp, Q&A sessions were held, allowing students to engage directly
with the instructors and clarify their understanding of the topics covered. These
interactive sessions provided valuable opportunities for students to deepen their
knowledge and resolve any doubts they had about the experiments and lectures.
In conclusion, the Tzu Chi University Biology Camp 2024 at Kuen Cheng High
School was a resounding success. It provided students with a unique opportunity to
engage with cutting-edge biological techniques, inspiring many to pursue further
studies in science. The combination of practical experience, theoretical learning, and
interactive Q&A sessions have left participants with a strong interest in learning
molecular biology and a newfound appreciation for the complexities of life at the
molecular level.